2.6

Consumables

1. Micro reaction tubes (2 ml).

2. Reaction tubes (15 and 50 ml).

3. Pipettes and tips (0.1–2.5, 2–20, 10–100, 100–100 μl).

4. 96-Well plates, flat bottom, clear and 12-well plates with clear

bottom, suitable for cell culture.

5. Microscope slides (e.g., SuperFrost plus microscope slides,

Fisher Scientific, Schwerte, Germany, cat. nr.: 10149870).

2.7

Hardware

1. Bioreactor perfusion platform as described by Schuerlein et al.

[14]

(Customized

from

University

Hospital

Wu¨rzburg,

Department for Tissue Engineering and Regenerative Medi-

cine, Wu¨rzburg, Germany). As an alternative, a CO2 cell cul-

ture incubator with accessibility to a peristaltic pump and space

for the bioreactor perfusion system could be used.

2. Set for bioreactor assembly: Sterile surgical gloves, coat and

sheets, non-absorbable braided suture material (strength 2-0),

surgical tweezers and needle holder.

3. Cell culture incubator with controlled CO2 atmosphere

(5–10% according to requirements of used media) and temper-

ature (37
C).

4. Sterile bench, large enough to assemble the perfusion system

(see Fig. 1).

Fig. 1 Complete perfusion setup placed inside the incubator platform with

controlled atmosphere and temperature. 1: Medium reservoir; 2: Medium sam-

ple syringes; 3: Roller pump; 4: 0.2 μm sterile filter for adding WST-1 cell

proliferation assay reagent; 5: 3D printed reactor containing vascular grafts; 6:

Attached syringes to collect WST-1 cell proliferation assay reagent

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209